Fig. 3: mtRNA activates the MAVS signaling pathway during CICD. | Nature Communications

Fig. 3: mtRNA activates the MAVS signaling pathway during CICD.

From: Executioner caspases restrict mitochondrial RNA-driven Type I IFN induction during chemotherapy-induced apoptosis

Fig. 3: mtRNA activates the MAVS signaling pathway during CICD.The alternative text for this image may have been generated using AI.

a Western blot taken from A375 CASP3-/-7-/- cell lysates with an additional CRISPR/Cas9 knockout against the indicated genes. The isogenic cell lines were treated with either DMSO or 0.5 µM PLX-4720 and 0.5 µM S63845 for 24 h. TLR3 protein is shown by the identified band on the blot. The data are representative of two independent experiments (n = 2). b A375 CASP3-/-7-/- cells with an additional knockout against the designated gene were treated under identical conditions as in (a), and RT-qPCR analysis was performed to measure ISG15 and IFIT3 transcript levels. Each dot represents a biological replicate, and the data are the results of three independent experiments (n = 3); one-way ANOVA with Tukey’s multiple-comparisons test, p-values are included in the figure; ns = not significant. Data are presented as mean ± SEM. mRNA levels were normalized to DMSO-treated control cells. c A375 CASP3-/-7-/- cells were treated with PLX-4720 (2 µM) and S63845 (2 µM) in the presence or absence of the TBK1/IKKε inhibitor Bay-985 (0.2 µM) for 24 h and western blots for the indicated proteins were performed on the lysates. d Western blot displaying specified proteins from A375 wild-type control or IRF3-/- cells following treatment with 2 µM PLX-4720 (PLX), 2 µM S63845 (S6), and 10 µM Emricasan for 24 h. e Western blot of A375 wild-type cells following 24 h treatment with the indicated combination of PLX-4720, S63845, Emricasan, and IMT1B. f Proposed mechanism for mtRNA signaling in apoptosis created with BioRender.com. c–e The data are representative of three independent experiments (n = 3).

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