Table 1 Cryo-EM data collection, refinement and validation statistics of HCA2-Gi complex
From: Structural insights into the human niacin receptor HCA2-Gi signalling complex
|  | HCA2-Gi complex (EMD-33241) (PDB 7XK2) |
|---|---|
Data collection and processing | Â |
Magnification | 105k |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 60 |
Defocus range (μm) | −1.0 ~ −2.0 |
Pixel size (Ã…) | 1.04 |
Symmetry imposed | C1 |
Initial particle images (no.) | 3685029 |
Final particle images (no.) | 273841 |
Map resolution (Ã…) | 3.1 |
   FSC threshold | 0.143 |
Map resolution range (Å) | 2.9–5.5 |
Refinement | Â |
Initial model used (PDB code) | 6N4B |
Model resolution (Ã…) | 3.1 |
   FSC threshold | 0.5 |
Map sharpening B factor (Å2) | −100 |
Model composition | Â |
   Non-hydrogen atoms | 8791 |
   Protein residues | 1128 |
   Ligands | 1 |
B factors (Ã…2) | Â |
   Protein | 77 |
   Ligand | 86 |
R.m.s. deviations | Â |
   Bond lengths (Å) | 0.004 |
   Bond angles (°) | 0.94 |
Validation | Â |
   MolProbity score | 1.72 |
   Clashscore | 5.94 |
   Poor rotamers (%) | 0.53 |
Ramachandran plot | Â |
   Favored (%) | 94.17 |
   Allowed (%) | 5.83 |
   Disallowed (%) | 0.00 |
