Fig. 2: lrNK cells control HCMV replication in vitro.
From: Control of human cytomegalovirus replication by liver resident natural killer cells
a Schematic of VDA. Seeded fibroblasts were infected with HCMV at ~0.01 MOI/well with sorted cNK or lrNK added after 24 h. After a further 7 days, cultures were fixed and immunostained for DAPI and HCMV-IE (secondary antibody on AF568). b Immunostained plates were analysed on a Hermes Wiscan scanning microscope with MetaMorph imaging software. Images were generated using tiles encompassing 50% of the well. c NK subset control of HCMV spread relative to infected cells without NK cells. Paired HCMV + (grey bars; individual donor n = 9) and HCMV- (open bars; individual donor n = 4) cNK and lrNK cell control of cell assoc. Merlin (left) and cell free Merlin (right). Data are shown as box and whisker plots showing line at median and min and max data points. Experiments were run once for each donor. Significance was assessed using two-tailed Wilcoxon’s signed-rank sum test (paired data) or a two-tailed Mann-Whitney U test (unpaired data) with P values less than 0.05 considered significant. Source Data are provided as a Source Data file.
