Fig. 3: Single-molecule manipulation of IF1-inhibited bMF1.
a Schematic images of the manipulation procedure. When bMF1 was stalled by IF1, the magnetic tweezers were turned on to stall bMF1 to rotate one clockwise or counterclockwise revolution at the rate of 0.5 revolutions per second (rps). After manipulation, released bMF1 either resumed its rotation (ON) or did not (OFF). These behaviors indicate whether or not IF1 dissociates from bMF1 under the stalling time. b Reactivation probability of IF1-inhibited bMF1 after manipulation. “Hyd” and “Syn” represent the direction of hydrolysis (counterclockwise) and synthesis (clockwise), respectively. Values represent reactivation probability (\({P}_{{ON}}\)) ± SD. \({P}_{{ON}}\) was defined as the probability of an ON event against total molecules. The SD of \({P}_{{ON}}\) is given as \(\sqrt{{P}_{{ON}}\,(100-{P}_{{ON}})/N}\), where \(N\) is the number of total molecules (N = 19-26 molecules). Source data and the exact number of molecules in each data point are provided as the Source Data file.
