Fig. 2: Complete replication cycles of sunY fragments.
From: Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment
a Illustration (left) and PAGE analysis (right) of separately performed template-dependent ligations of the three sunY fragments A123, B123, and C123. Lanes show reactions before (T0) and after 2 h of incubation. b–c Full replication cycle of fragment C123. Replication proceeds vial ligation of the RNA substrates for the sense strand (C1, C2, C3-Cy5, red) and the antisense strand (tC1-FAM, tC2, tC3, blue). In the AWI-systems, both the sense and antisense strands emerged b with the template present and c without the template present, suggesting that sunY partially acted as a template for antisense strand synthesis (gray box). Under isothermal conditions no products were observed after PAGE analysis. Source data are provided as a Source Data file.
