Fig. 4: Assembly of the IFT-A complex into the anterograde IFT train. | Nature Communications

Fig. 4: Assembly of the IFT-A complex into the anterograde IFT train.

From: Structural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train

Fig. 4

a Fitting of IFT-A into the cryo-ET density map of the anterograde train. The head and base cores are colored in light yellow and pale blue as Fig. 1e, and the flexible TPRs in the elongated and assembled states colored in pink and orange, respectively. b Close views of the inter-molecule interactions formed between IFT121ZBD and IFT139N (1), between IFT140WD and IFT121WD (2), between IFT144TPR and IFT140TPR (3-4), and between IFT144ZBD and IFT139N (5). c Fitting of atomic models of Tetrahymena IFT-A and Chlamydomonas IFT-B into the anterograde train map unveiling the putative [IFT-A]-[IFT-B] interfaces. IFT-A is from this study and colored as Fig. 1a, and IFT-B is from Lacey et al. with IFT172 (residues 1-1104) colored in cyan, IFT88 (residues 122-690) in yellow, IFT74 (residues 135-340) in orange and the rest of IFT-B in light brown. EMDB code of microtubule doublet (MTD) is EMD-20631. PDB codes of IFT-B, dynein-2 motor domain and tail domain are 8BD7, 6RLA and 6RLB, respectively. d Periodic arrangement of the anterograde train. Six IFT-B, three IFT-A and two dynein-1b in one periodic unit are colored as c, while the remaining is shown in grey.

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