Fig. 2: MHC-II⁺CD11c^–CD11b⁺ cell population is decreased in the colon of Clec7a^–/– mice.

a Apc^Min/+ and Apc^Min/+Clec7a^−/− mice were treated with 1% DSS for 7 days and were sacrificed 4 weeks later. Flow cytometric analysis was carried out to identify Dectin-1-expressing cells in colorectal polyps and non-polyp tissues. b, c Apc^Min/+ and Apc^Min/+Clec7a^−/− mice were treated with 1% DSS for 7 days and were sacrificed 4 weeks later. Proportions of T cells, B cells and DCs in total tissue-infiltrating cells (b, αβT cells, **P = 0.0020; γδT cells, **P = 0.0076; CD19⁺ B cells, **P = 0.0069; IgG⁺ B cells, **P = 0.0058; CD11c⁺ DCs, **P = 0.0014, ****P < 0.0001), and proportions of MHC-II⁺CD11c⁺ and MHC-II⁺CD11c^–CD11b⁺ cells in tissue-infiltrating CD45⁺ cells (c, MHC-II⁺CD11c⁺, **P = 0.0039, *P = 0.0157; MHC-II⁻CD11c⁻CD11b⁺, *P = 0.0563, ****P < 0.0001) in non-polyp tissues or polyps in the colon were examined by flow cytometry (n = 3/Apc^Min/+ group, n = 5/Apc^Min/+Clec7a^−/− group). d WT mice were treated with AOM-3DSS and were sacrificed after 12 weeks. Indicated types of cells in polyps and non-polyp tissues were purified with autoMACS and Il6 and Il1b expression was examined by RT-qPCR (n = 3 biologically independent samples/group). e WT mice were administrated with AOM followed by 3 cycles of DSS. After 11 weeks of AOM treatment, these mice were sacrificed and CD11b⁺ and CD11c⁺ cells were purified from colorectal polyps with autoMACS. The sorted cells were stimulated with an agonistic β-glucan OXCA for 16 h with or without pretreatment with an antagonistic β-glucan laminarin, for 3 h, then, Il6 and Il1b expression were determined by RT-qPCR (n = 4 biologically independent samples/group; in Il6 panel, ***P = 0.0002, *P = 0.0382; Il1b panel, ****P < 0.0001, **P = 0.0012). Data in a–e are representatives of two independent experiments. Data in b–e are expressed as means ± SD. Data in b, c are analyzed using two-way analysis of variance (ANOVA) and in e using one-way ANOVA followed by Tukey’s multiple-comparisons test. Source data are provided in the Source Data file.