Fig. 5: Decreased lipid accumulation due to adipose lipolysis dysfunction leads to compromised MIER1 regulation and impaired liver regeneration. | Nature Communications

Fig. 5: Decreased lipid accumulation due to adipose lipolysis dysfunction leads to compromised MIER1 regulation and impaired liver regeneration.

From: Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice

Fig. 5

a Immunoblot (HSL) in Control and Lipe-AKO adipose tissues. The liver triglycerides (TG) content (b) (n = 8, 7, 8, 6, 8 in Control group, and n = 8, 6, 6, 6, 8 in Lipe-AKO group, at different time points after PHx), liver/body weight ratio (%) (c) (n = 9, 8, 10, 10, 8 in Control group, and n = 9, 9, 8, 8, 9 in Lipe-AKO group, at different time points after PHx), immunoblots of liver MIER1 and EIF2S1 phosphorylation (d) in Control or Lipe-AKO animals before or after surgery. e Polysome profiles and qRT-PCR analysis of distributions of indicated transcripts in liver tissues collected before or 24 h after hepatectomy from Lipe-AKO animals. n = 3. The liver TG content (f) (Control, n = 10; Lipe-AKO, n = 7; Lipe-AKO; Mier1 sgRNA, n = 9), liver/body weight ratio (%) (g) (Control, n = 10; Lipe-AKO, n = 7; Lipe-AKO; Mier1 sgRNA, n = 9), liver Ki-67 immunostaining (h) (Control, n = 10; Lipe-AKO, n = 7; Lipe-AKO; Mier1 sgRNA, n = 9), and liver immunoblots (PCNA, Cyclin A2 and GAPDH) (i) in Control, Lipe-AKO or Lipe-AKO; Mier1 sgRNA animals at 36 h after hepatectomy. Values represent means with SEM. P values were assessed by unpaired, two-tailed Student’s t test (e), Two-Way ANOVA with post hoc Šídák’s multiple-comparison tests (b, c), or One-Way ANOVA with Dunnett’s multiple comparisons test (f, g, h). The experiments were repeated for three times with similar results (d, i). The average values of the quantification of western blots were indicated (d, i). Source data are provided as a Source Data file.

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