Fig. 10: Deafness-related mutation on PDZD7 affects the LLPS capacities of USH2 proteins.
From: Temporal and spatial assembly of inner ear hair cell ankle link condensate through phase separation
a PDZD7 PDZ1 is structurally superimposed with Harmonin PDZ1/SANS PBM29. Superimposition shows that G103 on PDZD7 PDZ1 corresponds to G104 on Harmonin PDZ1. G103 localizes on the binding groove and may be involved in the interaction with USH2A. b Summaries of the binding affinities between WT or deafness-related mutants of PDZD7 and USH2A-CT(S). N.D., not detectable. c GST pull-down assay showing that GST-WHRN could not pull down PDZD7 G103R in the presence of USH2A-CT. d Fluorescence images show that PDZD7 G103R was absent in the droplets formed by USH2 protein phase separation. Scale bar: 10 μm. WHRN NPDZ12 and PDZD7 PDZ12 were abbreviated as WHRN and PDZD7, respectively. PBM, PDZ binding motif. Source data are provided as a Source Data file.
