Fig. 2: Characterization of the interactions between PDZD7 with ADGRV1 and USH2A.
From: Temporal and spatial assembly of inner ear hair cell ankle link condensate through phase separation
a The schematic diagram shows the interactions among the four USH2 proteins. b Up panel, summaries of the binding affinities between WT or △PBM constructs of USH2A-CT(S) or ADGRV1-CT and different PDZD7 fragments; bottom panel, summaries of the binding affinities between PDZD7 PDZ12 and WHRN NPDZ12, ADGRV1-CT and USH2A-CT. c Binding affinities of USH2A-CT(S) to different PDZD7 fragments determined by ITC. Truncating PBM (△PBM) from USH2A-CT abolished the binding to PDZD7 PDZ12. d Binding affinities of ADGRV1-CT to different PDZD7 fragments determined by ITC. e GST pull-down assay showing that GST-PDZD7 PDZ12 pulled down Usherin-CT(S) but not VLGR1-CT. f GST pull-down assays show that GST-WHRN NPDZ12 pulled down USH2A-CT(S) and ADGRV1-CT. N.D., not detectable. The different PDZD7 fragments include PDZ1, PDZ2, PDZ2, PDZ12, or PDZ3. PBM, PDZ binding motif. Source data are provided as a Source Data file.
