Fig. 3: WHRN NTD dimerization contributes to the assembly of the ternary USH2 complex. | Nature Communications

Fig. 3: WHRN NTD dimerization contributes to the assembly of the ternary USH2 complex.

From: Temporal and spatial assembly of inner ear hair cell ankle link condensate through phase separation

Fig. 3: WHRN NTD dimerization contributes to the assembly of the ternary USH2 complex.The alternative text for this image may have been generated using AI.

a The schematic diagram shows that dimerized WHRN interacts with USH2A and ADGRV1 to assemble a ternary USH2 complex. b SEC-MALS showed the molecular sizes of WHRN, WHRN/USH2A-CT(S), WHRN/ADGRV1-CT, or USH2A-CT(S)/WHRN/ADGRV1-CT. The samples for fitting molecular sizes were eluted on a Superose 12 10/300 GL or Superdex 200 increase 10/300 GL column. c Summaries of stoichiometry in b. d Fractionation coupled with FPLC showed three bands on the gel, confirming the existence of WHRN, USH2A-CT(S), and ADGRV1-CT in the peak. WHRN NPDZ12 was abbreviated as WHRN. Source data are provided as a Source Data file.

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