Fig. 4: The assembly of the quaternary USH2 complex requires the dimerized USH2A-CT. | Nature Communications

Fig. 4: The assembly of the quaternary USH2 complex requires the dimerized USH2A-CT.

From: Temporal and spatial assembly of inner ear hair cell ankle link condensate through phase separation

Fig. 4: The assembly of the quaternary USH2 complex requires the dimerized USH2A-CT.The alternative text for this image may have been generated using AI.

a The schematic diagram shows that PDZD7 is recruited by dimerized USH2A to assemble the quaternary USH2 protein complex. b SEC-MALS showing the molecular size of USH2A-CT, summarized in Fig. 5c. c GST pull-down assay showing that GST-WHRN pulled down PDZD7 in the presence of USH2A-CT and quaternary protein complex was successfully assembled. d Co-sedimentation assay showed that WHRN, PDZD7, ADGRV1-CT, and USH2A-CT were enriched in the pellet. WHRN NPDZ12 and PDZD7 PDZ12 were abbreviated as WHRN, and PDZD7, respectively. Source data are provided as a Source Data file.

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