Fig. 2: CEP sensitivity depends on cytokinin perception via AHK2 and AHK3 in the root and shoot.
From: CEP peptide and cytokinin pathways converge on CEPD glutaredoxins to inhibit root growth
a Representative images and (b) relative primary root length for cytokinin perception mutants grown on medium with or without CEP3 peptide (10−6 M) for 10 days in comparison to Col-0 and cepr1-3 (n = 8 plants). c Sensitivity of ahk2,3 to increasing CEP3 concentrations (0, 10−8, 10−7 or 10−6 M) at 10 days of growth (n = 17-21 plants). Root length expressed as a percentage of seedlings grown on medium without CEP3 for each respective genotype. d Effect of CEP3 overexpression in wild-type and ahk2,3. Primary root length after 7 days growth for CEP3ox plants was normalised to their respective background control (Col-0 or ahk2,3) (n = 12 plants). e Primary root length for reciprocal hypocotyl grafts between CEP3 overexpressing plants in the Col-0 and ahk2,3 backgrounds (n = 15–22 plants). Primary root length measured 12 days post grafting. f Representative images and (g) relative primary root length of gain-of-function AHK mutants grown on medium with or without CEP3 peptide (10−6 M) for 12 days in comparison to Col-0. Letters in (b-e, g) indicate significant differences (ANOVA followed by Tukey HSD test, p < 0.05). Scale bars = 1 cm. Box plot centre line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range. See Supplementary Fig 2 for associated absolute root growth measurements and representative images. Exact p values and sample sizes for each treatment group are provided in the Source Data file.
