Table 1 List of T. brucei strains used in this studya
From: The RRM-mediated RNA binding activity in T. brucei RAP1 is essential for VSG monoallelic expression
Strain | Description | References |
|---|---|---|
TbRAP1F2H+/- | One F2H-tagged WT TbRAP1 and one deleted allele | |
PVS3-2/OD1-1 | WT TbRAP1 alleles, VSG9 is active | |
TbRAP1F/+ | One floxed allele and one WT TbRAP1 | |
TbRAP1F/∆MybL | One floxed allele and one N-terminally F2H- and NLS-tagged ∆MybL mutant | |
TbRAP1F/∆RRM | One floxed allele and one N-terminally F2H- and NLS-tagged ∆RRM mutant | current study |
TbRAP1F/2FA&5A | One floxed allele and one N-terminally F2H- and NLS-tagged F655AF694AR737AK738AR739AR740AR741A mutant | current study |
TbRAP1F/2FQ | One floxed allele and one N-terminally F2H-tagged F655QF694Q mutant | current study |
TbRAP1F/2FL | One floxed allele and one N-terminally F2H-tagged F655LF694L mutant | current study |
TbRAP1F/2FA | One floxed allele and one N-terminally F2H-tagged F655AF694A mutant | current study |
TbRAP1F/5A | One floxed allele and one N-terminally F2H- and NLS-.tagged R737AK738AR739AR740AR741A mutant | |
TbRAP1F/∆DB | One floxed allele and one N-terminally F2H- and NLS-tagged ∆DB mutant | |
TbRAP1-/2FL | One deleted allele and one N-terminally F2H-tagged F655LF694L mutant, derived from TbRAP1F/2FL cells by treating cells with Cre | current study |
