Fig. 10: Nanoparticle treatment reshapes immune microenvironment and potentiates the therapeutic effects of radiotherapy in PVPF8 murine gliomas.

C57 mice received intracranial implantation of 7.5×10⁴ PVPF8 cells, radiotherapy (RT, 3 Gy×3) and two administrations of saline or P-LNP/diABZI (2.5 mg/kg diABZI) through intravenous injections. a Histology of the brains was evaluated by H&E staining. Scale bar, 2.5 mm; inset scale bar, 500 μm. b Freshly dissected brains were analyzed by flow cytometry for immune composition, TAMC, and CD8⁺ T cell phenotypes. n = 5 mice. Statistics were determined by two-sided Student’s t-test; data are presented as mean + /- SEM. Representative dot plots of flow cytometric analysis are provided in Supplementary Fig. 20. c Survival curves of the PVPF8 tumor-bearing mice received RT (3 Gy×3) and two administrations of saline or P-LNP/diABZI (2.5 mg/kg diABZI) through intravenous injections. n = 9 mice. Statistics were determined by Log-rank method. Source data are provided as a Source Data file.