Fig. 3: Cr3+ rescues mitochondria from hyperglycaemia-induced fragmentation through targeting ATP5B. | Nature Communications

Fig. 3: Cr3+ rescues mitochondria from hyperglycaemia-induced fragmentation through targeting ATP5B.

From: Mitochondrial ATP synthase as a direct molecular target of chromium(III) to ameliorate hyperglycaemia stress

Fig. 3: Cr3+ rescues mitochondria from hyperglycaemia-induced fragmentation through targeting ATP5B.The alternative text for this image may have been generated using AI.

a Representative microscopic imaging of HepG2 cells with mitochondria stained by MitoTracker under normal glucose condition (5.6 mM, as Ctrl), high glucose-induced stress (40 mM, as HG) and high glucose-induced stress with Cr3+ supplementation (100 µM) (HG + Cr3+) (n = 10). Inset: 4 times enlargement of mitochondria. Scale bars: 5 µm. b, c Quantitative analysis and quantification of mitochondrial morphology illustrated by form factor (FF) and aspect ratio (AR). n = 10; mean ± SEM. Two-sided Student’s t test. d, e Quantitative analysis and quantification of mitochondrial morphology in scramble or ATP5B null (siATP5B-transfected) HepG2 cells. n = 5; mean ± SEM. Two-sided Student’s t test. Representative microscopic images are shown in Supplementary Fig. 11. Mitochondria remains fragmented even when 100 µM Cr3+ were supplemented to ATP5B-silenced cells. Individual data points (c, e) are available from Source Data, which are provided as a Source Data file.

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