Fig. 2: Compiled screening results and representative plate analysis.

Representative screening output scatterplots for the specific (A) and ReFrame (B) libraries, and for 2 single plates from the ReFrame primary screening, with NITD609 (C) and TD-6450 (D) as finally selected hits. In panel A, “K” refers to Kinase Inhibitors Box and “P” refers to Pathogen Box. Killing and retention rates are on the x and y axes, respectively. Negative controls (red squares), and positive controls including calyculin A 75 nM for stiffening activity (green triangles), Gentian violet 50 µM for killing effect (green circles) and NITD609 0.5 µM for combined stiffening and killing effect (green squares), fell inside and outside of the main cloud of inactive test compounds (empty gray circles) defined by linear regression +/−95% prediction bands SD (full line and dotted lines, respectively). Hits were either confirmed by DRA (Blue X), or not (blue empty triangles). Source data are provided as a Source Data file.