Fig. 6: Linkage specificity profiles of DUBs from different DUB families. | Nature Communications

Fig. 6: Linkage specificity profiles of DUBs from different DUB families.

From: Neutron-encoded diubiquitins to profile linkage selectivity of deubiquitinating enzymes

Fig. 6: Linkage specificity profiles of DUBs from different DUB families.The alternative text for this image may have been generated using AI.

a Heatmap showing the percentage of consumed diUb isoforms by 9 different DUBs (4.0 µM) from different DUB families after 180 min incubation. b Full quantification profiles of diUb disappearance over time for USP11, USP32, USP34 and RPN11/RPN8. An equimolar mixture (8 x 1.6 μM), containing all neutron-encoded diUb isoforms, was incubated with the DUB at 37 °C, samples were taken at the indicated time points and analysed by LC-MS (n = 1). Colors represent the differently linked diUbs as indicated. For full DUB characterization profiles see Supplementary Data 2c. Source data are provided as a source data file.

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