Fig. 5: TfCut-catalyzed PBAT degradation.
From: Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases
a The predicted binding mode of the polymer chain of PBAT in the active site tunnel of TfCut-DM. The polymer chain is drawn as BABTaB (cyan sticks). The catalytic triad, W195, and DM are colored in yellow, magenta, and blue, respectively. The zoom in figure is shown on the right side. The interaction network of BABTaB with surrounding residues in TfCut-DM is shown in magenta sticks and gray dash lines (<3.5 Å). The catalytic triad residues are shown in green sticks and the interactions are shown with orange dash lines. b The proposed method of TfCut-mediated PBAT degradation, including endo- and exo-cleavage. The representative PBAT polymer is drawn as the repeats of BABTaB and other combinations. The major intermediates that have TPA on the termini are depicted.
