Fig. 10: The HIDDEN cell compartment requires ELF3 expression. | Nature Communications

Fig. 10: The HIDDEN cell compartment requires ELF3 expression.

From: Single cell transcriptomic analysis of HPV16-infected epithelium identifies a keratinocyte subpopulation implicated in cancer

Fig. 10: The HIDDEN cell compartment requires ELF3 expression.The alternative text for this image may have been generated using AI.

a Summary of RELI (top) and HOMER (bottom) analysis indicating ELF3 hits in red (permutation-based strategies detailed in Methods.) Source data is provided in Supplementary Data 6. b The ELF3 motif was identified by HOMER and RELI analysis as a potential driver of HIDDEN cell formation. c Representative H&E images from n = 1 raft set generated from HPV16+ cells transduced with NTsh or ELF3sh constructs. Scale bar, 50 µm. d Representative IF image from n = 1 raft set of HPV16+ NTsh rafts and ELF3sh rafts validates a dramatic decrease in ELF3+ HIDDEN cells, localized on the surface epithelium, with ELF3 knockdown and elimination of the ELF3+ HIDDEN cell compartment suprabasal to the K10+ layer (representative of n = 5 frames across 1 set of rafts). Scale bar, 50 µm. e Western blot of 3D raft lysates shows increased ELF3 protein levels in HPV+ vs HPV16– rafts, and a decrease in ELF3 in ELF3sh knockdown vs NTsh control rafts. f Quantification of the number of ELF3+ cells per frame, (n = 5 per raft with lines representing mean values, two-way ANOVA with Dunnett’s multiple comparisons against NTsh, p < 0.0001 for ELF3sh-1 and ELF3sh-2). g RT-qPCR of HIDDEN cell biomarkers is consistently decreased in ELF3sh rafts compared to NTsh rafts (n = 3 rafts, two-way ANOVA with Dunnett’s multiple comparisons against NTsh, p < 0.0001 for ELF3sh-1 and ELF3sh-2). f, g Source data is provided in the Source data file.

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