Fig. 7: Nascent transcription and chromatin accessibility at DSB and meiotic recombination hotspots. | Nature Communications

Fig. 7: Nascent transcription and chromatin accessibility at DSB and meiotic recombination hotspots.

From: A-MYB and BRDT-dependent RNA Polymerase II pause release orchestrates transcriptional regulation in mammalian meiosis

Fig. 7: Nascent transcription and chromatin accessibility at DSB and meiotic recombination hotspots.The alternative text for this image may have been generated using AI.

a Metaplots (top) and heatmaps (bottom) of RPKM-normalized ATAC-seq signal centered on DSB hotspots inferred from SPO11 oligos for leptonema/zygonema (LZ), pachynema (P), and diplonema (D). Peaks are sorted in all prophase I substages by decreasing order of ATAC-seq signal intensity in leptonema/zygonema. b Metaplot (top) and heatmap (bottom) of H3K4me3 signal centered on DSB hotspots for LZ. c, d Metaplots of the average leChRO-seq signal centered on annotated TSSs (C) and SPO11 oligos (D) for LZ, P, and D. e Percentage of ATAC-seq peaks that are also transcribed based on observed overlap with dREG peaks (dot) and overlap of dREG peaks with randomly shuffled ATAC-seq peaks over 1000 iterations (violin plot). Statistical testing was performed with a one-sided Fisher’s exact test; p = 0.000999. f Observed overlap of DSB hotspots inferred from SPO11 oligo data with dREG and ATAC-seq peaks (dot) and with randomly shuffled dREG and ATAC-seq peaks (violin plot). Empirical p-value is reported. Statistical testing was performed with a one-sided Fisher’s exact test; p = 0.000999. Source data are provided as a Source data file.

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