Fig. 3: Ptbp1 knockout in pan-endothelial cells causes ventricular noncompaction.
From: Endothelial deletion of PTBP1 disrupts ventricular chamber development
a H&E staining showing the trabecular myocardium (TM) and compact myocardium (CM) in E13.5 Ptbp1fl/fl and Tie2-Cre;Ptbp1fl/fl heart sections. Scale bars, 100 µm. b Immunofluorescence staining of endomucin (EMCN) in E13.5 Ptbp1fl/fl and Tie2-Cre;Ptbp1fl/fl heart sections. The graph shows the quantification of the CM and TM thickness. n = 4 mice for Ptbp1fl/fl group and n = 5 mice for Tie2-Cre;Ptbp1fl/fl group. Scale bars, 100 µm. c H&E staining shows the TM and CM in E15.5 Ptbp1fl/fl and Cdh5-CreERT2;Ptbp1fl/fl mice heart sections. Scale bars, 100 µm. d Immunofluorescence staining of EMCN in E15.5 Ptbp1fl/fl and Cdh5-CreERT2;Ptbp1fl/fl heart sections. The graph shows the quantification of the CM and TM thickness in E15.5 Ptbp1fl/fl and Cdh5-CreERT2;Ptbp1fl/fl heart sections. n = 5 mice per group. LV left ventricle, RV right ventricle. Scale bars, 100 µm. The data are presented as the mean ± s.e.m. P values were calculated by unpaired two-tailed t test. Source data are provided as a Source Data file.
