Fig. 1: Characterization of PfENT1.
a Nucleoside binding by PfENT1 as measured by ITC. A representative ITC experiment is presented. The binding affinity (Kd) is presented as the value of the mean ± SD (n = 3), n means independent experiment. N.D., not detectable. Supplementary Fig. 2 shows the original ITC data and analyses. b The inosine transport activity of PfENT1. Time course for uptake of inosine in the knock-in yeast strain. The yeast strain without PfENT1 was tested as a control. Three independent experiments are performed for each point. Data are presented as mean ± SD. c Nucleoside competition and compound inhibition of PfENT1. The transport of [3H]-inosine was examined in uptake assays at 30 min in the presence of the indicated nucleosides/nucleobase (5 mM) and inhibitor GSK4 (10 μM). The yeast strain without PfENT1 was tested as a negative control. The PfENT1 knock-in yeast strain in the absence of non-radioactive nucleosides and inhibitor was test as positive control. Three independent experiments are performed for each substrate or inhibitor. Data are presented as mean ± SD. Source data are provided as a Source Data file.
