Fig. 5: Simulated temperature rise under cyclic and conventional holographic illumination. | Nature Communications

Fig. 5: Simulated temperature rise under cyclic and conventional holographic illumination.

From: Ultrafast light targeting for high-throughput precise control of neuronal networks

Fig. 5

a Temperature rise induced on an illuminated spot under conventional (black) and cyclic-illumination (blue), using a power per cell, \({P}_{{std}}\), of 10 mW and \({P}_{{cyc}}=\sqrt{H\cdot }\, {P}_{{std}}\)= \(\sqrt{H}\cdot 10\) mW, in conventional and cyclic-illumination, respectively; illumination \({{{{{{\rm{t}}}}}}}_{{dw}}=\) 10 ms; H = 20; 50 µs illumination pulses; 1 ms per each cycle b Volumetric distribution of 160 spots (H = 20 holograms, m = 8 spot per hologram) uniformly distributed in a 350 × 350 × 100 µm3 volume. c Temperature rise induced on a central spot when 160 spots are illuminated as depicted in (b) under conventional (black) and cyclic-illumination (blue). Inset: Temperature rise induced on the central spot by the 159 neighboring spots. d, e Temperature rise for different spots density d distributed in the considered volume under conventional (d) and cyclic-illumination (e). f Maximum temperature rise for cyclic- and conventional-illumination as simulated in (d, e). \({{{{{{\rm{t}}}}}}}_{{dw}}=\) 20 ms. Power per cell in conventional illumination \(P\left(z\right)=10\cdot {e}^{z/{{{{{{\mathscr{l}}}}}}}_{s}}\) mW; Power per cell in cyclic-illumination \(P\left(z\right)=10\cdot {e}^{z/{{{{{{\mathscr{l}}}}}}}_{s}}\cdot \sqrt{H}\); H = 20 in (bd).

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