Fig. 2: Impaired LrNK function contributes to early-Abx enhanced HCC progression. | Nature Communications

Fig. 2: Impaired LrNK function contributes to early-Abx enhanced HCC progression.

From: Early life gut microbiota sustains liver-resident natural killer cells maturation via the butyrate-IL-18 axis

Fig. 2: Impaired LrNK function contributes to early-Abx enhanced HCC progression.The alternative text for this image may have been generated using AI.

a Experimental design of c-myc/AKT/SB100-induced HCC model in control or early-Abx mice. b, c In vitro tumor imaging and liver /body weight was shown (n = 5 per group). d Representative FACS plots and bar graph for the expression (MFI) of IFN-γ and CD107a in LrNK cells from control and early-Abx HCC mice (n = 5 per group). e Representative FACS plots and bar graph for the cytotoxicity of LrNK cells from control or early-Abx HCC mice against CFSE-labeled YAC-1 cells (n = 5 per group). f Representative FACS plots and bar graph for the expression (MFI) of TIGIT and Tim3 on LrNK cells from control or early- Abx HCC mice (n = 5 per group). g, h Experiment scheme and survival curves of control and early-Abx mice injected with AKT/Myc/SB100 plasmids and treated with anti-asialo GM1 or anti-NK1.1(n = 5 per group). Dots represent data from individual mice, and error bars represent SEM per group in one experiment. Statistical significance was tested by two-tailed Student’s t test (c–f) or Long-Rank test (h). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, no significance. Source data are provided as a Source Data file.

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