Fig. 1: Endocytosis-related AP-2 and DrpC associate with K13 at peripheral micropore structures in T. gondii.
From: Stable endocytic structures navigate the complex pellicle of apicomplexan parasites
A Collapsed projections of 3D-SIM images of intracellular parasites within the host cell vacuole showing K13 (green) with five different AP-2 related proteins: (i) KAE, (ii) AP-2α, (iii) AP-2µ, (iv) AP-1/2β and (v) AP-2σ (all magenta). B K13 (green) with dynamin related protein DrpC (magenta). The IMC1 (blue) shows the parasite inner membrane complex, and zoomed panels show micropores either in side (s) or top (t) projections as indicated. Reporter epitopes and the terminus of fusion are shown in the figures. See Fig. S1 for wide-field fluorescence images and Fig. S2 for further examples. Scale bars for large and small panels are 2 µm and 0.2 µm, respectively. C Immuno-TEM against v5-tagged K13 showing gold labelling specific to the classic micropore structure. See Fig. S3 for further cell examples.
