Fig. 3: The micropore occurs in openings of the IMC at the alveolar plate boundaries. | Nature Communications

Fig. 3: The micropore occurs in openings of the IMC at the alveolar plate boundaries.

From: Stable endocytic structures navigate the complex pellicle of apicomplexan parasites

Fig. 3

A 3D-SIM images of intracellular parasites showing K13 (green) with a suture protein ISC3 (magenta). *The suture lines were extrapolated in the rightmost image based on the ISC3 signal. B Top and side 3D-SIM projections of micropores with markers for the IMC outer and inner membrane proteins GAP45 (i–ii) and GAPM1a (iii–iv), respectively; IMC subpellicular network protein IMC1 (v–vi); and GPI-anchored surface protein SAG1 (vii–viii) (all in magenta). Yellow arrows correspond to structures shown in the magnified panels, and all scale bars are 2 µm and 0.2 µm for the overview and zoomed images, respectively. C Model of the endocytic K13 micropore complex in the pellicle of T. gondii tachyzoite from these data and Figs. 1 and 2. IMC inner membrane complex. See Figs. S5 and S6 for further examples.

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