Table 1 Sequences of guide RNAs utilized in this work

From: GalNAc-Lipid nanoparticles enable non-LDLR dependent hepatic delivery of a CRISPR base editing therapy

Target*

Protospacer (5’−3’) #

gRNA sequence (5’−3’) #

LDLR

gaaatgcatctcctacaagt

gsasasaugcaucuccuacaaguguuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcusususu

LDLR

gggactcatcagagccatcc

gsgsgsacucaucagagccauccguuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcusususu

ANGPTL3

aagatacctgaataactctc

asasgsauaccugaauaacucucguuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcusususu

  1. # letters a, c, g, u and t indicate adenosine, cytidine, guanosine, uridine and thymidine nucleotides; (b) the lowercase letter s indicates phosphorothioate (PS) backbone.
Back to article page