Fig. 2: KDM2A is dispensable for non-ALT cells. | Nature Communications

Fig. 2: KDM2A is dispensable for non-ALT cells.

From: Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

Fig. 2: KDM2A is dispensable for non-ALT cells.

a–c Competition-based proliferation assays of the indicated sgRNAs in IMR90-T (a), HeLa (b), or NCI-H1299 cells (c). A GFP reporter is linked to sgRNA expression. sgCtrl and sgPCNA were included as a negative or positive control, respectively. Data were expressed as mean ± s.e.m. of three independent experiments; two-tailed paired t-test. d, e Clonogenic assay (d) and KDM2A western blot analysis (e) of sgCtrl, sgK#1, or sgK#2-transduced IMR90-T cells. Crystal violet staining was conducted on day 22 post-seeding. f, g Clonogenic assay (f) and KDM2A western blot analysis (g) of sgCtrl, sgK#1, or sgK#2-transduced HeLa cells. Crystal violet staining was conducted on day 15 post-seeding. h, i Clonogenic assay (h) and KDM2A western blot analysis (i) of sgCtrl, sgK#1, or sgK#2-transduced NCI-H1299 cells. Crystal violet staining was conducted on day 15 post-seeding. j Western blot analysis of KDM2A and ACTB in whole-cell lysates prepared from indicated HeLa cell lines. The KDM2A-depleted lines (clone#1–7) were established from clonally isolated HeLa cells transduced with sgK#1. The sgCtrl-transduced HeLa line was included as a control. SE short exposure, LE long exposure. Source data are provided as a Source Data file.

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