Fig. 5: Ex vivo eRIC uncovers many novel RBPs.
From: The RNA-binding protein landscapes differ between mammalian organs and cultured cells
a For each organ, the number of eRIC hits that are annotated RBPs, that bear a known RNA-binding domain (RBD), and that are novel RBPs (i.e., not previously reported as RBP or as bearing an RBD, and not identified in any published study). Right: Number of RBPs identified in organs or cell lines that are (metabolic) enzymes. b Upset plot representing the number of RBPs (y-axis) shared between this study and published lists of mouse and human RBPs (only intersections comprising 25 or more RBPs are shown). a, b Pink, novel RBPs. c Protein class annotation of the novel RBPs identified in any of the three organs studied (based on PANTHER protein class ontology22). Note that compared to the overall RBP dataset (see Fig. 2c), the class “translational protein” is under-represented, while the category “Metabolite interconversion enzyme” is over-represented. d The presence of the identified novel RBPs in total proteomes of ten different cell lines employed in previous RBP profiling studies was interrogated using public data (see methods). The number of novel RBPs (y-axis) identified in inputs across an increasing number of cell lines (x-axis) is shown.
