Fig. 7: Enzyme-RBPs broadly interact with nucleotide cofactors.
From: The RNA-binding protein landscapes differ between mammalian organs and cultured cells
a Enzymes of intermediary metabolism (metabolite interconversion enzymes in PANTHER protein class22) identified as eRIC hits in the brain, kidney, or liver (designated Enzyme-RBPs) were classified based on their catalytic activity; for comparison, the same classification was applied to enzymes of intermediary metabolism that were expressed in at least one organ but were not identified as eRIC hits. b Catalytic activity enrichment analysis among the enzyme-RBPs identified in mouse organs (Fisher’s one-tailed test without correction for multiple comparison). The upper and bottom panels show, respectively, the main types and corresponding subtypes of enzymatic activities (indicated by the same distinct color in both panels). c, d Bar graphs indicating the proportion of enzymes expressed in organs and identified (pink) or not (gray) as eRIC hits that bear typical nucleotide-binding domains (any nucleotide, p.adj = 4.29e-4; NAD(P), p.adj = 4.63e-2; AMP, p.adj = 1.18e-2) (c) or employ specific cofactors (any nucleotide, p.adj = 8,77e-4; CoA, p.adj = 6.01e-4) (d). *p.adj <0.05, ***p.adj <0.001, #p.adj = 0.065 testing specifically nucleotide cofactors in (d) (Fisher’s exact test with Benjamini–Hochberg correction).
