Table 1 PCR primers used for this study
From: DAXX drives de novo lipogenesis and contributes to tumorigenesis
RT-qPCR primers (5’ to 3’) | |
|---|---|
ActinB-F-Real | GCTCCTCCTGAGCGCAAGTACTC |
ActinB-R-Real | GTGGACAGCGAGGCCAGGAT |
Daxx-RT-F | GAGGCGTCTCTCCTCACAAC |
Daxx-RT-R | TCTCATGCACTGACCTTTGC |
SREBP1-F | CTGCTGTCCACAAAAGCAAA |
SREBP1-R | GGTCAGTGTGTCCTCCACCT |
SREBP2-F | ATCGCTCCTCCATCAATGAC |
SREBP2-R | TTCCTCAGAACGCCAGACTT |
FASN-F | CACAGGGACAACCTGGAGTT |
FASN-R | ACTCCACAGGTGGGAACAAG |
HMGCR-F | GTCATTCCAGCCAAGGTTGT |
HMGCR-R | CATGGCAGAGCCCACTAAAT |
STARD4-F | GGCGAGTTGCTAAGAAAACG |
STARD4-R | CCCTGGGCGTATATGGTCTA |
HMGCS1-F | GGGACACATATGCAACATGC |
HMGCS1-R | CACTGGGCATGGATCTTTTT |
FDPS-RT-F | CCAAGAAAAGCAGGATTTCG |
FDPS-RT-R | CCGGTTATACTTGCCTCCAA |
ChIP primers | |
FASN-prom-F1 | TAGAGGGAGCCAGAGAGACG |
FASN-prom-R1 | GCTGCTCGTACCTGGTGAG |
ACACA-prom-F1 | CAAGGGAAATTGAGGCTGAG |
ACACA-prom-R1 | CGTTCCAGGAGCATCTGATT |
FASN-ChIP-3’UTR-F1 | GTGAACCATGACTGCACCAC |
FASN-ChIP-3’UTR-R1 | GAGCCCTCGGTGACATACAT |
ACACA-ChIP-3’UTR-F1 | TCATGGCCAAACTGTTGAAA |
ACACA-ChIP-3’UTR-R1 | TGGGGTCCATTGTTTCTGAT |
SREBF-prom-F2 | TCCTTTAAACAAGGCGGAGA |
SREBF-prom-R2 | TCAGCAGCTCAGATTTGCAT |
Primers for amplifying a fragment in the SREBF2 promoter | |
SREBF2-prom-F2 | CAGCTGAAGCTTGCATGCCTGCAGGTAGGCAGCTGGGAAGATGA |
SREBF2-prom-R2 | GAGTATATATAGGACTGGGGATCCGTGAGGGTCTCCATGGTCTC |
