Fig. 6: Pulmonary IRF4-dependent cDC2s are required to promote pulmonary type-2 immune responses in pre-patent murine schistosome infection.

CD11cΔIrf4 mice were percutaneously infected with 180 cercariae, and samples taken at d21. A Representative flow cytometry plots show depletion of lung MGL2⁺ IRF4 dependent cDC2s. Gate frequencies show % of CD11b⁺ cDC2s. B BAL cell isolates were assessed via flow cytometry for macrophages, eosinophils, neutrophils, B cells, CD4⁺ and CD8⁺ T cells. C Lung cell isolates were stimulated with PMA/ionomycin, and cytokine production assessed via flow cytometry. Data are from 3 independent experiments (n = 18 biologically independent animals). Data were fit to a linear mixed effect model, with experimental day as a random effect variable, and groups compared with a two-sided LS means Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.