Fig. 1: The cryo-EM structure of human AE2 in an inward-facing conformation.
From: The structural basis of the pH-homeostasis mediated by the Cl−/HCO3− exchanger, AE2
a The cell-based function assay on hAE2. The intracellular pH changes upon the recovery of extracellular Cl− were recorded for the Expi293 cells overexpressing the human SLC4A2 gene in the presence or absence of DIDS inhibitor, with the cells without overexpression as the blank control. The results were shown as mean ± s. d. of experiments in triplicate. b, c hAE2acidic-KNO3 cryo-EM map as viewed parallel to the cell membrane. The electron density corresponding to two protomers in dimeric hAE2 was shown in blue (chain A) and pink (chain B), respectively. The non-protein electron density surrounding the transmembrane regions (TM) was shown in gray, except for the electron density of CHS, colored in orange. d The cartoon representation of hAE2 monomer topology. e The dimeric structure of the hAE2. The hAE2 structure was shown as cartoon model and viewed at the same angle as (b). The membrane region was shown as gray background. f The cartoon model of the hAE2 dimer as viewed from the extracellular side of the cell membrane. Protomer B was colored in pink, and protomer A was colored to the same scheme as shown in (d).
