Fig. 4: Fermentation profile of DHB-producing strain TW1356. | Nature Communications

Fig. 4: Fermentation profile of DHB-producing strain TW1356.

From: Construction of a synthetic metabolic pathway for biosynthesis of 2,4-dihydroxybutyric acid from ethylene glycol

Fig. 4

The strain was cultivated in defined mineral medium supplemented with glucose for growth. The working volume of the bioreactor was 1 L (a). At 1.5 h after pathway induction by addition of IPTG (0.5 mM), repeated addition of 10 mM glycolaldehyde (GA) was started at intervals of ~2 h (b) and product formation was monitored (c, d). A balance for GA-derived carbon is shown for the end of the fed-batch fermentation at 24 h (e). Results are the mean of two biological replicate experiments. Error bars correspond to the standard deviation of the mean. Source data are provided as a Source Data file.

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