Fig. 5: Bioconversion of EG to DHB.
Results are shown for incubation times of 48 h using growing (a, b) and resting cells (c, d). a Impact of EG concentrations on DHB biosynthesis by exponentially growing cells of strain TW363 (derivative of MG1655 ΔyqhD ΔaldA). b Impact of deleting the DHB dehydrogenase LldD on EG to DHB conversion. EG was added at a final concentration equal to 320 mM. a, b Exponentially growing cells of strains TW363 and TW1828 (derivative of MG1655 ΔyqhD ΔaldA ΔlldD) were grown at 37 °C, 220 rpm in 25 mL mineral medium supplemented with LB at 10% (v/v) with a starting OD = 0.2. IPTG (0.5 mM) and EG were added when OD600 reached ~0.6. Results represent the mean of two biological replicate experiments. Individual data points are shown as coloured black dots. c Impact of medium composition on conversion of 320 mM EG to DHB using resting cells of strain TW1828. Fermentation profile of EG bioconversion in LB medium is shown in (d). c, d Cells were grown in 200 mL mineral medium supplemented with LB at 10% (v/v) with a starting OD = 0.2. IPTG (0.5 mM) was added when OD600 reached ~0.6. After 6.5 h, cells were concentrated to OD600 = 15 in 5 mL of indicated medium containing EG and IPTG (0.5 mM) and incubated at 37 °C, 220 rpm. Where indicated, addition of one glucose feed-bead was carried to facilitate the controlled release of the sugar (with a feed rate of ~0.5 mM or 0.09 g L−1 per hour, corresponding to a total added concentration of 25 mM or 4.5 g L−1). Results are expressed as mean of two biological replicates. Error bars represent the standard deviation of the mean. Source data are provided as a Source Data file.
