Fig. 1: Bioguided fractionation of Corema album L. leaf PEF extract identifies genipin derivatives as the bioactive compounds. | Nature Communications

Fig. 1: Bioguided fractionation of Corema album L. leaf PEF extract identifies genipin derivatives as the bioactive compounds.

From: Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage

Fig. 1: Bioguided fractionation of Corema album L. leaf PEF extract identifies genipin derivatives as the bioactive compounds.The alternative text for this image may have been generated using AI.

a PEF was fractionated by LC with a methanol gradient (pointed purple line, right y-axis). The chromatogram was obtained by quantification of total phenols (dashed gray line, left y-axis; μg GAE.mL-1: gallic acid equivalent per mL) for the fractions of C. album. L. A total of 16 fractions were obtained and tested in the yeast PD model. Control cells carrying the empty vector and cells expressing two copies of αSyn-GFP were incubated in galactose medium supplemented or not with 30 μg GAE.mL-1 for each fraction for 24 h. OD600nm was monitored every hour. The bioactivity is measured by the area under the curve (AUC). E vs A, *p = 0.0202, F1 vs A, *p = 0.0102, F6 vs A, *p = 0.0289, (n = 3) b Structure of genipin, an iridoid monoterpene compound. c Growth curves of αSyn-GFP yeast in the presence or not of genipin. Growth curves were treated using R package grofit to adjust a nonlinear parametric regression (model-based) and the growth parameters were estimated from the best model fit. d Final biomass and AUC 95 % confidence intervals were used to assess statistical differences between conditions and are represented as horizontal lines. Control cells with empty vector– E, αSyn-GFP cells – A, αSyn-GFP cells +10 μM genipin – AG.

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