Fig. 1: CircRNA levels increase in RMS.

a Volcano plots showing, for each circRNA identified in the RNA-Seq experiment, the log₂ fold change and the −log₁₀ p value either in the comparison between RD (left panel) or RH4 (right panel) cell lines and wild-type myoblasts. The distributions of the fold change values are shown above the volcano plots. Significantly altered circRNAs (p value < 0.05) or unaltered circRNAs are indicated by red or gray dots, respectively. b Stacked bar charts with percentage of down- and upregulated linear (“LinRNAs”), circRNAs (“CircRNAs”), top 20% expressed circRNAs (“CircRNAs TOP”) or “high-confidence” circRNAs (“CircRNAs HC”) in the comparison between RD and wild-type myoblasts (left panel) or between RH4 and wild-type myoblasts (right panel). P values for the differences between proportions were calculated using Fisher exact two-tailed test. c Scatter plots showing, for each circRNA identified in the RNA-seq experiment, the log₂ fold change along with that of its cognate linear RNA, in the comparison between RD (left panel) or RH4 (right panel) cell lines and wild-type myoblasts. Significantly deregulated circRNAs are indicated by blue dots when their linear counterpart is deregulated in the same direction (“Concordant”) and by red dots when the linear is either unaltered or deregulated in the opposite direction (“Discordant circ”); significantly deregulated linRNAs are indicated by light gray dots when their circular counterpart is unaltered (“Discordant lin”); unaffected circRNAs are indicated by dark gray dots when their linear counterpart is not altered (“Unaltered”). d Cumulative distribution function of the circular versus the linear isoform expression (“CLR”) in myoblasts (red) and RD (blue, left panel) or RH4 (blue, right panel). P values for the differences between cumulative distribution functions were calculated using Kolmogorov–Smirnov two-tailed test. Source data are provided as a Source Data file.