Fig. 3: K19 is specific to immature MHCII^neg stages of TEC development.

a Representative FACS plots showing expression of UEA1 and Ly51 by total EpCAM1⁺ cells, K19^-EpCAM1⁺ cells and K19⁺EpCAM1⁺ cells at E15.5, and corresponding quantitation (n = 12, from 3 independent experiments). Data analysed using a Student’s t-test. b Representative FACS plots showing expression of MHCII and CD80 to define mTEC^neg (MHCII^-CD80^-), mTEC^int (MHCII^intCD80^int) and mTEC^hi (MHCII^hiCD80^hi), and the corresponding expression of K19 by these subsets (upper panel), and expression of Aire within mTEC^hi, and the corresponding expression of K19 by Aire⁺ mTEC^hi (lower panel). Bar chart shows proportion of K19⁺ cells within mTEC^neg, mTEC^int, and mTEC^hi at E15.5 (n = 12, from 3 independent experiments). Data analysed using a one-way ANOVA, with Bonferroni post hoc test. c Representative FACS plots showing expression of MHCII within UEA1^-Ly51⁺ cells, and expression of K19 by these subpopulations. Bar chart shows proportion of K19⁺ cells within MHCII^-UEA1^-Ly51⁺ and MHCII⁺UEA1^-Ly51⁺ TEC at E15.5 (n = 12, from 3 independent experiments). Data analysed using a Student’s t test. The data are shown as mean ± SEM.