Fig. 1: Tools and datasets used for m6A mapping based on the ONT DRS platform.
From: Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing
a Pipeline and strategies employed by various m6A mapping tools. Information on the requirement for a negative control, minimum coverage and motif specificity for each tool is shown in brackets. “√” and “x” indicate the requirement for a negative control, while “-” indicates that a negative control is optional. “N” indicates a lack of motif limitations, while “D” and “R” indicate DRACH and RRACH, respectively. Note that MINES detects m6A in AGACT and GGACH motifs. b m6A detection and quantification by LC-MS/MS using WT and Mettl3 KO samples. c Violin plots of ONT data features for WT and KO samples, the upper and lower limits represent the 75th and 25th percentiles, respectively, while the center line represents the median; upper and lower whiskers indicate ±1.5× the interquartile range (IQR). The number of reads is indicated in parentheses. d Metagene distribution plot with motif preferences of m6A peaks identified from the MeRIP-seq data.
