Fig. 6: Detection sensitivity for m6A sites varies under different coverage and stoichiometry.
From: Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing
a, b Relative recall rates (a) and precision (b) for m6A sites under different coverage. Relative recall rates/precision were calculated as the ratio of the recall rate/precision for m6A sites with a given coverage to the overall mean (across all sites). The validation set was derived from the miCLIP results. The same validation set is also used in c. c Recall rates and precision of ELIGOS and Tombo_com for m6A detection under different sequencing coverage. d–f Relative recall rates for m6A sites with different methylation rate/site enrichment. Relative recall rates were calculated as the ratio of the recall rate for m6A sites with a given methylation rate/site enrichment to the overall mean (across all sites). The validation sets were derived from the m6A-REF-seq (d), miCLIP (e) and miCLIP2 (f) results.
