Fig. 2: Investigation of the lubrication of PRG4 and tryptase β-processed PRG4.
From: Tryptase β regulation of joint lubrication and inflammation via proteoglycan-4 in osteoarthritis
a Schematic of the experimental workflow of the lubrication test (created with BioRender). b Lubrication of tryptase β (grey), PRG4 (initial mass of PRG4 1 μg) (blue), PRG4 + tryptase β for 18 h (1:100 enzyme/substrate) (magenta), PRG4 + tryptase β + 1 mM AEBSF incubated for 18 h (1:100 enzyme/substrate) (green). The error bars denote the error of the mean ± standard deviation as obtained from 5 independent experiments. c Lubrication of PRG4 (initial mass of PRG4 1 μg) (blue), PRG4 + tryptase β incubated for 10 min (green), 30 min (orange), 1 h (pale blue), 6 h (purple), 24 h (black) and tryptase β (grey). The error bars denote the error of the mean ± standard deviation as obtained from 5 independent experiments.
