Fig. 6: Single-cell analysis of polyfunctional CD8+ D4-IgG4-CD28TM CAR T cells at the mRNA and protein levels. | Nature Communications

Fig. 6: Single-cell analysis of polyfunctional CD8+ D4-IgG4-CD28TM CAR T cells at the mRNA and protein levels.

From: The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer

Fig. 6

a Flowchart of the single-cell protein and RNA expression analysis platform. The illustration was partially created with BioRender.com. b Identification of two CAR T clusters, low polyfunctionality and high polyfunctionality, in 23 CD8+ D4-IgG4H-CD28TM CAR T cells. c Functional analysis of two distinct CAR T clusters. d The corresponding RNA sequencing data reveals unique gene expression profiles for these two CAR-T clusters. e REACTOME pathway analysis of unique genes in (c). The CAR T cells used in this figure were produced using the donor 2’s PBMCs. Source data are provided as a Source Data file.

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