Fig. 5: Sublethal doses of borrelidin A slow the emergence of cephalosporin resistance in wildtype E. coli. | Nature Communications

Fig. 5: Sublethal doses of borrelidin A slow the emergence of cephalosporin resistance in wildtype E. coli.

From: Collateral sensitivity profiling in drug-resistant Escherichia coli identifies natural products suppressing cephalosporin resistance

Fig. 5: Sublethal doses of borrelidin A slow the emergence of cephalosporin resistance in wildtype E. coli.The alt text for this image may have been generated using AI.

A Ceftazidime MIC values as determined through sequential passaging of wildtype E. coli MG1655 over twelve days under increasing ceftazidime drug pressure, either alone (red; Cef), with an increasing concentration of norfloxacin (black; Nor), with a constant concentration of 1 at 32 µM (light blue), or with a constant concentration of 1 at 128 µM (blue). Populations that reached 50% growth under the highest concentration of ceftazidime for each treatment condition were used to inoculate the following day’s cultures. Dotted line represents the EUCAST breakpoint for ceftazidime resistance in E. coli. Data shows the mean of three independent passaging experiments (n = 3); error bars denote the standard error of the mean (SEM). B On the 12th day, strains from each passaging condition were tested with three cephalosporins (ceftazidime, cefadroxil, cefaclor), two fluoroquinolones (norfloxacin and ciprofloxacin), and 1 in triplicate. Values indicate average fold-change MICs compared to wildtype across three independent experiments (n = 3). Source data are provided as a Source Data file.

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