Fig. 5: CAR affinity and tumor antigen density regulate the degree of NFAT activation. | Nature Communications

Fig. 5: CAR affinity and tumor antigen density regulate the degree of NFAT activation.

From: Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor models

Fig. 5: CAR affinity and tumor antigen density regulate the degree of NFAT activation.The alternative text for this image may have been generated using AI.

a Lentiviral vectors encoding C215-iGFP, UBS54-iGFP and Y6V-iGFP CARs. b Flow cytometric quantification of CAR-mediated NFAT activation, as indicated by NFAT-driven GFP expression in Jurkat CAR-T cells following stimulation for 24 h with target cells. CD69 was used as a marker for T-cell activation. Numbers indicate the percentages of live, single cells in the GFP- or CD69-positive gate. Neg, low, mid and high represent negative-, low-, medium-, and high-EpCAM expression, respectively. c Relative mean fluorescence intensity (MFI) of GFP and CD69 expression from b. Data were normalized to PMA/Ionomycin treatment and were presented as mean ± SD of quadruplicate samples.

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