Fig. 3: KIAA1199 KO mice exhibit increased bone mass and bone strength. | Nature Communications

Fig. 3: KIAA1199 KO mice exhibit increased bone mass and bone strength.

From: KIAA1199 deficiency enhances skeletal stem cell differentiation to osteoblasts and promotes bone regeneration

Fig. 3: KIAA1199 KO mice exhibit increased bone mass and bone strength.The alternative text for this image may have been generated using AI.

A–H Bone mass was determined by µCT-scanning of trabecular bone (A, B, E, F) and cortical bone (C, D, G, H) on proximal tibia of 8 weeks male (A–D) and female (E–H) KIAA1199 knockout (KO) and corresponding wild-type (WT) mice. Photomicrographs of representative µCT-3D scan are shown in (A, C, E, G), and quantitative data analysis are shown (B, D, F, H). Trabecular bone volume per tissue volume (Tb. BV/TV), trabecular thickness (Tb. Th., mm), trabecular number (Tb. N., /mm3), trabecular connectivity density (Conn-dens., /mm3), trabecular structure model index (SMI), trabecular separation (Tb.Sp., µm), cortical (Ct) BV/TV, Ct thickness (Ct. Th., mm), n = 8. Scale bar (A, C, E, G) = 400 µm. I Three-point bending test was measured on femur of KO and WT male mice and quantitated by maximum load (N), ultimate stress (MPa), bone stiffness (N/mm) and failure energy (KJ), n (WT) = 11, n (KO) = 9. At least two sets animal’s experiments were detected and confirmed for each test, the results from one set experiment were presented. Data is pressed as means ± SD, statistical difference was determined by two-tailed unpaired Student’s t-test between two groups. *P < 0.05. **P < 0.01 and ***P < 0.001. Source data are provided as a Source Data file.

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