Table 1 Experimentally characterised designed Nb.b201 variants
From: Automated optimisation of solubility and conformational stability of antibodies and proteins
Nb.b201 variant | Tm (°C) | AMS50% (M) | CIC RT (min) | ΔTm (°C) | ΔRT (s) |
|---|---|---|---|---|---|
WT | 70.1 ± 0.05 | 0.76 in (0.75,0.79) | 11.859 ± 0.003 | 0 | 0 |
A31D | 72.7 ± 0.1 | 1.25 in (1.20,1.28) | 11.543 ± 0.014 | 2.6 ± 0.1 | −19.0 ± 1.1 |
H53P | 70.5 ± 0.05 | 0.94 in (0.82,1.19) | 11.889 ± 0.001 | 0.4 ± 0.05 | 1.8 ± 0.1 |
H53R | 71.3 ± 0.05 | 1.40 in (1.31,1.42) | 12.033 ± 0.002 | 1.2 ± 0.05 | 10.4 ± 0.3 |
T55D | 76.6 ± 0.3 | 1.37 in (1.16,1.47) | 11.399 ± 0.003 | 6.5 ± 0.3 | −27.6 ± 0.0 |
T55G | 80.7 ± 0.1 | 1.22 in (1.04,1.35) | 11.588 ± 0.003 | 10.6 ± 0.1 | −16.2 ± 0.0 |
T100K | 74.7 ± 0.1 | 1.47 in (1.45,1.48) | 11.736 ± 0.001 | 4.6 ± 0.1 | −7.4 ± 0.1 |
T100R | 73.1 ± 0.05 | 1.25 in (1.07,1.39) | 11.932 ± 0.002 | 3.0 ± 0.05 | 4.4 ± 0.3 |
A31D T55D | 78.5 ± 0.1 | 1.27 in (1.09,1.42) | 11.045 ± 0.001 | 8.4 ± 0.1 | −48.8 ± 0.2 |
A31D T55G | 82.9 ± 0.2 | 1.49 in (1.45,1.51) | 11.204 ± 0.001 | 12.8 ± 0.2 | −39.3 ± 0.2 |
H53P T55G | 80.4 ± 0.1 | 1.61 in (1.58,1.65) | 11.872 ± 0.002 | 10.3 ± 0.1 | 0.8 ± 0.3 |
A31D H53P T55G | 82.0 ± 0.1 | 1.18 in (1.08,1.22) | 11.444 ± 0.003 | 11.9 ± 0.1 | −24.9 ± 0.4 |
A31D H53P T55G T100K | 83.7 ± 0.1 | 1.19 in (1.06,1.38) | 11.120 ± 0.001 | 13.6 ± 0.1 | −44.3 ± 0.2 |
