Fig. 2: scRNASeq profiling of liver allografts. | Nature Communications

Fig. 2: scRNASeq profiling of liver allografts.

From: Immune cell dynamics deconvoluted by single-cell RNA sequencing in normothermic machine perfusion of the liver

Fig. 2

A Overview of the scRNASeq workflow. The proportions of epithelial cells (KRT18), leukocytes (PTPRC/CD45), and endothelial cells (SPARC) in the obtained scRNASeq dataset are indicated. B Uniform manifold approximation and projection (UMAP) plot of 118,448 single cells, color-coded by cell type. C Relative cell-type composition in liver tissues from eight individual patients. D UMAP plots, color-coded for the expression of indicated cell-type specific marker genes (red arrowheads). E Gene-expression levels of cell-type specific markers. F The proportion of leukocyte populations in liver tissue as determined by scRNASeq vs flow cytometry. G UMAP plot colored by the number of transcripts per cell. The color scale is clipped at 20,000. H Boxplot of the transcript count per cell-type. The values denote the average per patient (n = 8). The central line denotes the median. Boxes represent the interquartile range (IQR) of the data, whiskers extend to the most extreme data points within 1.5 times the IQR.

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