Fig. 3: Impact of NMP on immune cells within the liver. | Nature Communications

Fig. 3: Impact of NMP on immune cells within the liver.

From: Immune cell dynamics deconvoluted by single-cell RNA sequencing in normothermic machine perfusion of the liver

Fig. 3

A UMAP plot of 90,404 single cells (only T0 and T1 samples), color-coded by cell type. B Relative cell type composition in liver tissues pre (T0) and at the end of (T1) NMP. C UMAP-plot of 90,404 single-cells, color-coded by time-point [pre (T0) and at the end of (T1) NMP]. The monocyte/macrophage (1) and neutrophil (2) clusters are highlighted. D Multiplex Immunofluorescence images of immunology markers presented alone and together with pan-Cytokeratin and the phenotyping map pre (T0) and at the end of (T1) NMP. The phenotyping map was generated in InForm, each color dot represents a phenotype of the cell, black dots: other cells of unknown phenotype. Images are displayed at ×20 magnification (scale bar: 100 µm). E, F Cell densities (number of cells/mm2) for individual immunology markers in 10 patients. The upper panel E presents the values for each individual liver pre (T0) and at the end of (T1) NMP. The lower panel F presents a column statistical analysis for each biomarker (n = 10, paired t-test, two-tailed, mean ± SEM, **p = 0.0097).

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