Fig. 8: Phenotyping of perfusate immune cells and inflammatory profile during NMP. | Nature Communications

Fig. 8: Phenotyping of perfusate immune cells and inflammatory profile during NMP.

From: Immune cell dynamics deconvoluted by single-cell RNA sequencing in normothermic machine perfusion of the liver

Fig. 8: Phenotyping of perfusate immune cells and inflammatory profile during NMP.The alternative text for this image may have been generated using AI.

Perfusates collected at various time points during NMP (n = 26 livers subjected to NMP) were assessed for their dynamic changes of the proportions of A CD4+ T cells, B CD8+ T cells, C subtypes of CD3+ T cells with regulatory properties, D CD56+ NK cells, E CD45+CD14+ monocytes/macrophages, F CD45+HLA-DRlow granulocytes, and G dendritic cells. Graphs show the marginal effects. The values are estimated using linear regression analysis. The p-values refer to the change over time. The Least-Squares Means computed using a linear model is shown together with the 95% CI. N = 26 biologically independent samples. H Gene expression levels of indicated pro-inflammatory factors (interleukins/chemokines) in monocytes/macrophages and neutrophils pre (T0) and at the end of NMP (T1) as assessed by scRNASeq in eight donor livers. I Spectrum of assessed pro-inflammatory interleukins/chemokines produced by monocytes/macrophages and neutrophils and elevated on protein level in perfusate samples collected over perfusion time of 26 donor livers. (J) Gene expression levels of IL-6 and TNF in monocytes/macrophages and neutrophils pre (T0) and at the end of NMP (T1) as assessed by scRNASeq in transplanted (n = 6) and discarded (n = 2) livers. The central line denotes the median. Boxes represent the interquartile range (IQR) of the data, whiskers extend to the most extreme data points within 1.5 times the IQR. In addition, the perfusate of 26 donor livers subjected to NMP was measured for IL-6 and TNF levels at 1, 4, 6, 12 and 24 h of NMP and differences between transplanted (n = 18) and discarded (n = 8) livers were calculated. Graphs show the marginal effects. The values are estimated using linear regression analysis. The p-values refer to the change over time. The Least Squares Means computed using a linear model are shown together with the 95% CI. N = 26 biologically independent samples. Source data are provided as a Source Data file. Tcm: central memory T cells, Tem: effector memory T cells, Temra: effector memory cells re-expressing CD45RA T cells, DN T cells: double-negative T cells (CD4− and CD8-), NK cells Natural killer cells, DCs dendritic cells.

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